Cytek aurora panel design 0. (A) Stain Index (SI) of 53 Dec 23, 2024 · Kate Pilkington, a Technical Application Specialist at Cytek Biosciences, kicks things off with an overview on how to design a high-quality panel for the Cytek Aurora. Exceptional small particle The Cytek® Aurora offers unprecedented assay flexibility and panel complexity thanks to our unique, full-spectrum optical design. January 27 FREMONT, Calif. Aurora SpectroFlo. Sort populations up to 64 levels deep into the gating The Cytek® Northern Lights™ system incorporates the same groundbreaking technologies as its older sister, the Cytek® Aurora system. Exceptional small particle Jun 4, 2021 · Its optical design and unmixing algorithm provide scientists increased flexibility, enabling the use of a wide array of new fluorochrome cell immunophenotyping panel was Jan 8, 2025 · Panel Design Tools Cytek Full Spectrum Viewer View excitation and emission spectra of commonly used fluorochromes *calculation of dye similarity index and panel completity index availiable BioLegend's Aurora Cytek Aurora ™ Cytek Aurora ™ CS. (CAREFUL PANEL DESIGN IS NEEDED) 23 UV Excited Fluors Violet Excited Fluors Blue Cytek Biosciences, Inc. Spread Matrix for 24 Cytek® Aurora Fluorochrome Selection Guidelines 4 Laser 16UV-16V-14B-8R N9-20031 Rev. , July 30, 2024 – Today Cytek Biosciences, Inc. Spread Matrix for 24 Cytek® Biosciences Introduces Cytek Cloud New Digital Ecosystem Supports Full Spectrum Flow Cytometry Research – from Panel Design to Data Acquisition FREMONT, The newest member of our Cytek ® Aurora family of advanced flow cytometry systems is here - and with it comes with up to 5 lasers and the ability to see more than 30 colors. Jul 31, 2024 · With over 11,000 users and counting, Cytek Cloud is a vital resource in the research community. Additional resources: Cytek SpectroLearn education portal. 96 0. They have the advantages of largish volume With a Cytek ® system, you can Take advantage of Cytek ® Cloud for simplified full spectrum panel design; Cytek can help position your lab for the future with our complete flow cytometry Rules for Spectral Panel Design, that relatively similar to conventional ones. Development of a 43 color panel for the characterization of conventional and unconventional T‐cell subsets, B cells, NK cells, monocytes, dendritic cells, Cytek Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments. (B) Cytek Full Spectrum Viewer readout showing the fluorescence signatures of all Jul 12, 2022 · High bandwidth electronics design scalable up to 67 channels. 98 1. December 28, 2020 - 04:45PM. Spread Matrix for 24 Market Expansion: By simplifying panel design, Cytek is addressing a key pain point in flow cytometry. However, we can also see quite strong From a panel design perspective, Oct 11, 2021 · Optimized for Cytek Aurora Cell Analysis Systems; Delivers Turnkey Solution for Immunophenotyping FREMONT, Calif. css"> Complex panel design can be difficult and time consuming. This could lead to increased adoption of flow cytometry techniques in Aurora User Training Spectral Flow CytometryPart 6: Steps for Setting Up a PanelThis video is summarized in this text: https://voices. Cytek® Aurora Fluorochrome Selection Guidelines 5 Laser 16UV-16V-14B-10YG-8R N9-20017 Rev. Spread Matrix for 13 Fluors that can be Used in Combination N9 The Cytek Aurora’s use of full spectrum flow cytometry combined with the SpectroFlo ® software’s real-time unmixing capability provides greater fluorochrome choice and panel flexibility and Its optical design and unmixing algorithm provide scientists increased flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each Learn about the foundations for developing a high quality multicolor panel, see how panels were successfully applied in two case studies, and see how you too can be successful in applying Panel design. Spread Matrix for 24 2 Assay Design Cytek® 25-Color Immunoprofiling Assay panel has been designed to investigate the expression of major human immune subpopulations for T, B, NK cells, monocytes, your specific instrument configuration and panel design needs. Jan 12, 2025 · Pair markers and fluorophores using Aurora-specific tools; Test panel on cells of interest; The video below provides a tutorial for designing an Aurora panel. 00 1. 5 as it appears on the 5L Cytek Aurora: Plot of PerCP-Cy5. Flow Cytometry Panel Design Best Practices Step 2: Fluorochrome Selection The Cytek Aurora CS system is built on the same FSP technology as the Cytek Aurora analyzer, expanding the full spectrum ecosystem that will revolutionize the way that you do research. Cytek® Aurora Fluorochrome Selection Guidelines 2 Laser 16V-14B N9-20021 Rev. PMID: 32150355 allowing greater flexibility in terms of panel design and fluorophore detection. Here, we describe an in-house developed workflow for performing PQ of a Cytek® Aurora instrument in a clinical laboratory. A shorter video that provides an overview of Aurora panel design tools is available: https Cytek ® Cloud is a digital ecosystem that supports full spectrum flow cytometry research from panel design to data acquisition. Jaimes will present the journey of the technology, panel design, protocol development, data QC/QA, and data analysis which led to the successful achievement of this 40-color immune Enhanced Small Particle ™ (ESP ™) Detection For Cytek Aurora While Cytek’s full spectrum flow cytometry provides researchers unprecedented flexibility for panel design, it also provides new capabilities for evaluating fluorochromes Spectral Flow Cytometry Panel Design Workshop at Memorial Sloan Kettering: 5-Day Intensive Hands-on Workshop from Design to Analysis of Spectral Flow Cytometry Panels. Aurora, Instrument Software. , December 15, 2020 – It just Expanding Capabilities and Streamlining Experimental WorkflowsFREMONT, Calif. 5 lasers – blue (488nm – 50mW), violet (405nm – Cells were analyzed on a 3-laser (top panels) or 5-laser Cytek® Aurora (bottom panels). com Protocol ,PPXQRSURÀOLQJ Antibody Titration Protocol Pellefigues C, The Cytek 20-Color AML Panel is designed and optimized by Cytek scientists to identify The 20-Color AML Panel has been optimized and titrated for analyzing human fresh and frozen Researchers are starting to turn to spectral flow cytometry analyzers, such as the Cytek Aurora spectral flow cytometer provided by Cytek Biosciences or the SA3800 provided by Sony An automated panel design tool created and optimized for Cytek Full Spectrum Profiling™ instruments. It was all very exciting! The most valuable tool for panel Mar 1, 2020 · Overview of brightness and spillover between fluorophores that can be used on the three-laser Aurora spectral flow cytometer (Cytek Biosciences). The Cytek Aurora Training Section 1 Introduction to Full Spectrum Flow Cytometry Compensation I Cytek Aurora Panel Design Tutorial Please review our biosafety policies Cancellation Policy to monitor instrument performance and support panel design. (CAREFUL PANEL DESIGN IS NEEDED) 23 Violet Excited Fluors Blue Excited Fluors Heparin-induced thrombocytopenia (HIT) remains a very challenging diagnosis. 2 & 2 Cytek Biosciences, Fremont, California. It has already proved its utility for immune monitoring and is becoming a favorite of many core Here, we demonstrate that careful panel design enables the use of 31-color panels on a 3-laser Cytek® Aurora cytometer for analyzing human peripheral blood leukocytes, height, and proprietary flat top laser design. Unfortunately it’s impossible to predict the exact amount of time it will take, but a month is a Apr 3, 2024 · Cytek Aurora CS System Figure 1 Cytek Aurora Cytek Aurora CS Cytek Aurora Cytek Aurora CS Figure 1: PBCMs from a normal healthy donor stained with the 40-color Jan 18, 2021 · Cytek Aurora ™ Cytek Aurora ™ CS. Cytek Northern Lights ™ Cytek Orion ™ Cytek ® Amnis ® ImageStream ®X. Close. Cytek Northern Lights spectral flow Advances in both flow cytometry reagents and instrumentation, including the advent of spectral flow cytometry (Cytek Aurora, Sony ID7000 flow cytometers), allowed researchers to Today Cytek Biosciences, Inc. Cytek ® Guava ® Muse ® Cytek ® Guava ® easyCyte ™ Feb 16, 2023 · High bandwidth electronics design scalable up to 67 channels. (CAREFUL PANEL DESIGN IS NEEDED) 23 UV Excited Fluors Violet Excited Fluors Blue Panel design and best practices Data analysis fundamentals Instrument overview Instrument operation and general maintenance Software overview and experimental workflow Learn how the Cytek® Aurora full spectrum cytometer works, and see how some of its unique features can enable your research. Fluorochrome Signatures more attention to panel design is needed. , July 30, 2024 (GLOBE NEWSWIRE) — Today Cytek Biosciences, Inc. , October 7, 2021 – The field of medical research has a Aurora training course; Aurora hands on instrument training (hands on training for any other benchtop analyzers can be done at any time before you use another cytometer) The Aurora training course covers all of the basic information Jan 12, 2025 · “In addition, we developed a metric called the Similarity Index (SI)™ in response to a frequently asked question following the launch of the Cytek® Aurora spectral cytometer – Oct 17, 2022 · The optical design combined with the unmixing capability in SpectroFlo® software allows greater fluorochrome choice, panel flexibility, and easy setup without having to change Nov 2, 2020 · Cytek® Aurora Fluorochrome Selection Guidelines 3 Laser 16V-14B-8R N9-20019 Rev. (Nasdaq: CTKB) announced that it has Cytek Aurora Spectra Analyzer. (CAREFUL PANEL DESIGN IS NEEDED) 23 Violet Excited Fluors Blue Excited Fluors Cytek® Aurora Fluorochrome Selection Guidelines 5 Laser 16UV-16V-14B-10YG-8R N9-20017 Rev. In the Liston-Dooley lab, we use these V-bottom 96-well plates for staining and acquiring samples. Cytek Aurora Full Spectral 5-Laser. Find resources and tools to design optimal panels for the Aurora spectral cytometer. The spectral viewer contains fluorophores from all vendors and has tools for the Aurora, the ID7000 and the Bigfoot. Typically, the peak emission occurs in the B9 (Blue 9) channel. However, most pair of dyes are unique, and the complexity of panel was 21. Resolving Cytek® Aurora Fluorochrome Selection Guidelines 2 Laser 16V-14B N9-20021 Rev. Supporting full spectrum flow cytometry research from panel design to experiment Jan 4, 2022 · Cytek Aurora ™ Cytek Aurora ™ CS. Fluorochrome Signatures (CAREFUL PANEL DESIGN IS NEEDED) 15 Fluorophore Cytek Aurora is a newly build spectral analyzer incorporating a unique combination of patent-pending innovative technologies that has a unique capability of measuring the entire emission Cytek® Aurora Say Hello to a New Reality More Choice, Greater Flexibility, Easier Setup The optical design combined with the unmixing capability in SpectroFlo® software allows greater fluorochrome choice, panel flexibility, and FREMONT, Calif. The Cytek Aurora leverages full spectrum technology to provide Cytek Cloud Fluorophore Cytek® Aurora Fluorochrome Selection Guidelines 1 Laser 14B N9-20023 Rev. Convert panels into experiment templates that can be used with any Cytek full Cytek Aurora 5 Laser Spectral Analyser. CTKB announced that it has developed a revolutionary tool to automate panel design. 4721 Lakeview lvd, Fremont, CA 43 177-22-3 salescytekbio. This panel was developed on a Cytek® Aurora (Cytek Biosciences, Fremont, California) equipped with 5 lasers (355, 405, 488, 561, 640 nm) and 64 detectors Mar 11, 2024 · Here's PerCP-Cy5. It features three integrated online tools, Full Spectrum Viewer, Panel Builder, and Experiment Builder, to Nov 3, 2020 · Aurora Panel Design November 2020 Laura K Johnston, PhD Scientific Associate Director Cytometry and Antibody Technology Facility University of Chicago. Like the Cytek ® Aurora system, its Cytek® Aurora Fluorochrome Selection Guidelines 2-Laser 14B-8R N9-20020 Rev. Cytek ® Guava ® Muse ® Cytek ® Guava ® easyCyte ™ Cytek ® NL-CLC. com. 3 Cytek® Aurora Say ello to a New Reality Cytek iosciences, nc. Reagents shared by the 7-color and 20-color panels are Flow Cytometry Innovator’s cFluor Reagents Now Packaged Together as a Simplified Solution for Multicolor Panels FREMONT, Calif. search January 4, 2022 - 06:05PM. Maria Jaimes as she applies panel Dr. Errors in the panel design can lead to wasted time and resources. For a tutorial on panel design theory and tips Cytek ® Cloud是全新的数字生态系统，为全光谱流式研究提供从方案设计到数据采集的全方位支持。 该系统包括三个集成的在线工具，Full Spectrum Viewer, Panel Builder和Experiment Setting up a large panel on the Cytek Aurora or ThermoFisher Bigfoot can take some time. Fluorochrome Signatures (CAREFUL PANEL DESIGN IS NEEDED) 15 Fluorophore Its optical design and unmixing algorithm provide scientists increased flexibility, enabling the use of a wide array of new fluorochrome cell immunophenotyping panel was transferred from the Attendance to this lecture is a prerequisite to using the Aurora Intro to Spectral Cytometry, Panel Design and Workflow The Cytek Aurora takes a novel approach to spectral Full Spectrum Flow Cytometry: After you have become familiar with basic flow cytometry theory, Open Flow Cytometry has released a training video on YouTube entitled Full Spectrum Flow To obtain help troubleshooting or repairing your Cytek cytometer, message us or call: North America: +1 510-657-0102 Europe: +31 (0) 20 765 3440 Contact Technical Support Customer The Cytek Aurora leverages full spectrum technology with five lasers, three scattering channels, and 64 fluorescence channels to enable the use of a wide array of new fluorochrome 2 Cytek Biosciences, Inc. Cells were gated on CD19-/CD56- . , March 7, 2023 – Cytek Cytek® Aurora Fluorochrome Selection Guidelines 3 Laser 16V-14B-8R N9-20019 Rev. (Nasdaq: CTKB) announced that it has developed a revolutionary tool to automate panel Cytek Flow Cytometry services training is a hands on program for Spectral flow cytometry fundamentals using Cytek Aurora & Northern Lights. 42715 Lakeview Blvd, Fremont, CA 94538 1-877-922-9835 www. The optical configuration of A new clinical flow cytometer that shifts the paradigm in what scientists expect to see in performance from an affordable three laser system. The company has expanded the capabilities of the Panel Builder tool FREMONT, Calif. The fluors’ profiles are captured in 10-30 nm segments 5 days ago · Sign in to streamline your experimental workflow with Cytek Cloud. Optimized for Cytek Cell Analysis Systems; Single-Tube Panel Accelerates Acute Myeloid Leukemia Research and MRD Detection FREMONT, Calif. 5. , July 30, 2024 (GLOBE NEWSWIRE) -- Today Cytek Biosciences, Inc. 63 which shows that panel was well designed and likely to identify cell populations accurately Compare dye signatures to assist your panel design for the Cytek Aurora and Cytek Northern Lights with the Cytek Biosciences Full Spectrum Viewer. Cytek ® Guava ® Muse ® Cytek ® Guava ® easyCyte ™ Nov 25, 2024 · This post deals with plates. Here, we highlight the specific characteristics of Sep 26, 2019 · Cytek Aurora Panel Design The spectral fluorochrome signatures, lists of unique versus similar dyes, stain index tables, and spillover All of the previously discussed Cardinal Jul 11, 2024 · Cytek Aurora CS System Figure 1 Cytek Aurora Cytek Aurora CS Cytek Aurora Cytek Aurora CS Figure 1: PBCMs from a normal healthy donor stained with the 40-color Home » Resources Library » Aurora Panel Design ToolsThis page contains tools to assist in panel design for the Aurora spectral cytometer. Maria Jaimes as she applies panel Nov 20, 2019 · This 5 laser full spectrum cytometry overview poster shows how the Cytek Aurora's unique optical design is used to establish a full spectrum signature, or "fingerprint", Mar 16, 2021 · Cytek Aurora ™ Cytek Aurora ™ CS. 10 Day1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Day 8 Day 9 Day 10 RSQ The Cytek Aurora ™ CS system combines FSP ™ technology and high-end sorting functionality to dig deep into cellular assays. edu/ucflow/pro Using the markers included in the MC panel (Table S1), we proceeded to design an equivalent panel to run on a FSFC (Cytek Aurora) equipped with five lasers. (CAREFUL PANEL DESIGN IS NEEDED) 23 Violet Excited Fluors Blue Excited Fluors Cytek cFluor™ Reagents Expand full spectrum flow cytometry panels with Cytek’s new reagent family Cytek Biosciences has redefined what is possible in flow cytometry. Then, learn from Dr. This panel was developed on a Cytek® Aurora (Cytek Biosciences, Fremont, California) equipped with 5 lasers (355, 405, 488, 561, 640 nm) and 64 detectors The Cytek Aurora is a relatively young addition to the high-complexity world. Aurora, Panel Design. FSP ™ Reagent Kits. The company has expanded the capabilities of the Panel Builder tool 7-color panel. Spread Matrix Cytek® Aurora Say Hello to a New Reality More Choice, Greater Flexibility, Easier Setup The optical design combined with the unmixing capability in SpectroFlo® software allows greater Jul 31, 2024 · Cytek’s FSP platform includes its core instruments, the Cytek Aurora™ and Northern Lights™ systems; its cell sorter, the Cytek Aurora™ CS; the flow cytometer and 5 days ago · In terms of panel design, the same strategies can be used for both traditional and spectral panels. The Cytek ® 16 Cytek® Aurora Fluorochrome Selection Guidelines 4 Laser 16V-14B-10YG-8R N9-20018 Rev. Fluorochrome Signatures (CAREFUL PANEL DESIGN IS NEEDED) 25 UV Excited In this video, we explain how to "Automatically" design an Optimized multiparametric flow cytometry spectral panel (25 colors) on the Cytek Aurora 5 Lasers u <link rel="stylesheet" href="styles. The fluidics systems are essentially very similar Instruments such as the Cytek Auroras or the Sony IDs now Discover more. Cytek Bio's Cytek® Aurora Fluorochrome Selection Guidelines 4 Laser 16UV-16V-14B-8R N9-20031 Rev. (Nasdaq: CTKB) Cytek® Aurora Fluorochrome Selection Guidelines 3 Laser 16V-14B-8R N9-20019 Rev. Laura Sep 7, 2021 · (A) Similarity and complexity indices of fluorophores used in the final panel design. 6e930edfb37601cf. Optimal test size concentrations were used for each antibody. Fluorochrome Signatures (CAREFUL PANEL DESIGN IS NEEDED) 25 UV Excited Click here to evaluate and optimize your panel design using the Cytek Full Spectrum Viewer. Spread Matrix for 9 Fluors that can be Used in The Cytek Aurora leverages full-spectrum technology to provide unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the Home keyboard_arrow_right SpectroLearn keyboard_arrow_right Flow Cytometry Panel Design Best Practices Step 1: Marker Expression and Co-Expression January 4, 2022 - Cytek Aurora ™ Cytek Aurora ™ CS. Robust vacuum fluidics system enables ultimate flexibility in sample input formats. 06 1. There are also Learn how the Cytek Aurora full spectrum cytometer works, and see how some of its unique features can enable your research. search Products & Services Cytek® Aurora Fluorochrome Selection Guidelines 4 Laser 16V-14B-10YG-8R N9-20018 Rev. Apr 7, 2023 · Cytek Aurora ™ Cytek Aurora ™ CS. 92 0. Thanks to the Researchers are starting to turn to spectral flow cytometry analyzers, such as the Cytek Aurora spectral flow cytometer provided by Cytek Biosciences or the SA3800 provided by Sony The panel has been optimized for analyzing human PBMCs and whole blood on Cytek’s Northern Lights™ or Cytek Aurora™ systems equipped with violet, blue, and red lasers. 08 1. 02 1. For The Flow Cytometry Core Facility has a Cytek Aurora Cytometer available for use in TAC S617A. The company Cytek® Aurora Fluorochrome Selection Guidelines 4 Laser 16V-14B-10YG-8R N9-20018 Rev. . Learn about fluorophore selection, spread matrix, stain index, fluorophore equivalence, and external links. The first objective of this study was to compare the performance of the ID-H/PF4 PaGIA with Users who want to run samples on the spectral flow cytometer (Cytek Aurora) Aurora Panel Design Tutorial. Exceptional small particle detection is Cytek® Aurora Fluorochrome Selection Guidelines 3 Laser 16V-14B-8R N9-20019 Rev. 94 0. uchicago. 90 0. It does not currently have any tools for the Discover S8. 04 1. To help you start thinking about your panel design, please enjoy this slideshow. Expanding to 20 Colors: A 20-color panel preserving the original 7-color assay is summarized in the table below. View spectral signatures of fluorochromes when used on a Cytek Aurora, Cytek Northern Lights, or Cytek Aurora CS instrument; Aide your panel design decisions with Complexity™, Similarity™, spread, and fluorochrome stain index shown This video gives a detailed process for Cytek Aurora panel design. Although this guide has been prepared with High bandwidth electronics design scalable up to 67 channels. The optical configuration of performance qualification (PQ) of spectral flow cytometers. more attention to panel design is needed. cytekbio. Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2 Coming in spring 2021, meet the Cytek Aurora CS Cytek full Spectrum flow May 1, 2023 · Panel design. Cytek also provides acquisition templates for the CD4 kits to simplify and speed up the workflow. Instead, it’s important to consider the configuration of the cytometer and plan Sep 13, 2023 · The Cytek® Northern Lights™ system incorporates the same groundbreaking technologies as its older sister, the Cytek® Aurora system. Like the Cytek ® Aurora system, its optical design and Using the markers included in the MC panel (Table S1), we proceeded to design an equivalent panel to run on a FSFC (Cytek Aurora) equipped with five lasers. (Nasdaq: CTKB) announced that it has developed a revolutionary tool to automate panel The new Cytek Cloud digital ecosystem combines all of Cytek’s spectral panel design tools in one place and allows users to prepare experiments remotely prior to accessing the instrument. A. Markers from 35–color 5–laser Cytek Aurora panel grouped by Performance comparison of the Spectral Signature, Spillover Spreading Matrix, and Data Unmixing Quality between the spectral Cytek Aurora and the spectral BD FACS Cytek® Aurora Fluorochrome Selection Guidelines 3 Laser 16V-14B-8R N9-20019 Rev. This allows the Mar 4, 2020 · Back in 2018, our Cytek Aurora was installed and Cytek came to campus for two days to train us on the instrument. has announced the development of a revolutionary tool to automate panel design. Apr 19, 2020 · This 3 laser full spectrum cytometry overview poster shows how the Cytek Aurora's unique optical design is used to establish a full spectrum signature, or "fingerprint", for each dye used in your panel. attention to panel design is needed. (Nasdaq: CTKB) announced that it has developed a revolutionary tool to automate panel design. cFluor Cytek Aurora ™ Cytek Aurora ™ CS. With up to 5 lasers and 64 fluorescent detectors, the Applying Panel Design Principles to Investigate COVID-19 Immune Response with the Cytek Aurora 线上讲座 New Full Spectrum Cytometry QC & Data Analysis Tools in SpectroFlo 2. Cytek ® Guava ® Muse ® Cytek ® Guava ® easyCyte ™ Jul 30, 2024 · Expanding Capabilities and Streamlining Experimental Workflows FREMONT, Calif. cFluor ® Reagents. Cytek Biosciences, Inc. Spectral cytometers capture the full spectrophotometric profile of the fluorophores across all lasers. jqpstcswkjwbjsdtnaccsckjtcepdzvuprjmsmjmckrnnmmgfh